SERVICES>
1)
Determination of molecular mass of proteins and peptides by MALDI-TOF
2)
Protein identification by fingerprinting
- Enzymatic digestion (automatic or manual) with trypsin of proteins obtained from SDS-PAGE 1D or 2D gels. Digestion can be also carried out in-solution.
- Analysis of the digest by mass spectrometry (MALDI-TOF) in order to provide a fingerprinting spectrum
- Search in databases to identify the protein
3)
Protein identification by fingerprinting and peptide fragmentation by MALDI tandem Time-Of-Flight mass spectrometry (MALDI-TOF/TOF)
· Enzymatic digestion (automatic or manual) with trypsin of proteins obtained from SDS-PAGE 1D or 2D gels. Digestion can also be carried out in-solution.
· Analysis of the digest by mass spectrometry (MALDI-TOF) in order to provide a fingerprinting spectrum.
· Automatic peptide sequencing (1 to 3 peptides) (MS/MS).
· Search and identification of a protein by fingerprinting (data MS), by peptide sequencing (data MS/MS) or combined analysis (MS +MS/MS).·
- Automatic search and identification by GPS (Global Proteome Server) system connected to MALDI-TOF/TOF instrument
4) Peptide Sequencing
· Peptide sequencing by MALDI-TOF/TOF mass spectrometer
· Peptide sequencing by HPLC-Q-TOF. Soon available!!.
